Merlin Antikörper (pSer518)

Produktnummer ABIN117953

Der Kaninchen Polyklonal Anti-Merlin-Antikörper wurde für WB und EIA validiert. Er ist geeignet, Merlin in Proben von Human und Maus zu detektieren.

Kurzübersicht für Merlin Antikörper (pSer518) (ABIN117953)

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Merlin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Merlin Antikörper

Bindungsspezifität: pSer518

Spezifität: This affinity purified antibody is directed against human NF2 (Neurofibromatosis 2 gene product). This phospho-specific polyclonal antibody is specific for phosphorylated pS518 of human NF2 (neurofibromatosis 2 gene product). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross-reactivity with NF2 (Merlin) occurs in mouse tissue.

Aufreinigung: Affinity purified from monospecific antiserum by immunoaffinity chromatography. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.

Immunogen: Human NF2 (Merlin) phospho-peptide corresponding to a region of the human protein surrounding S518 and conjugated to Keyhole Limpet Hemocyanin (KLH).

Anwendungsinformationen

Applikationshinweise: This phospho-specific polyclonal antibody was tested by immunoblotting and ELISA. ByELISA, the antibody was found to be reactive with the phosphorylated form of theimmunizing peptide and minimally reactive with the nonphosphorylated form of theimmunizing peptide. Immunoblotting will detect human and mouse NF2 (Merlin). Althoughnot tested, this antibody is likely functional in immunohistochemistry andimmunoprecipitation. This product has been assayed against 0.1 g of phosphorylated peptide in a standardcapture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,000 to 1: 4,000 is suggested for this product. Less than 5 % cross-reactivity was detected against the nonphosphorylated form of theimmunizing peptide. Dilute the antibody 1: 1,000 for immunoblotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.24 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.

Details zu Merlin

Target: Merlin (NF2) (Neurofibromin 2 (NF2))

Andere Bezeichnung: Merlin

Hintergrund: The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence results in the occurrence of multiple tumors of the nervous system, particularly schwannomas and meningiomas. Merlin's similarity to ERM's suggests that it might share functions, acting as a link between cytoskeletal components and the cell membrane. The NF2 protein is highly expressed in human fibroblasts and is detected as a single band of about 75 kDa. This antibody is specific for the phosphorylated form of human NF2 (Merlin).Synonyms: Moesin-ezrin-radixin-like protein, NF2, Neurofibromin-2, SCH, Schwannomerlin, Schwannomerlin, Schwannomin, Schwannomin

Gen-ID: 4771

NCBI Accession: NP_000259

UniProt: P35240

Pathways: Cell-Cell Junction Organization